Where are the ATP and NADPH Used?

  • The products of light reaction are ATP, NADPH and O3 Of these, O2 diffuses out of the chloroplast while ATP and NADPH are used to drive the processes leading to the synthesis of sugars. This is the biosynthetic or dark phase of photosynthesis. This process does not directly depend on the presence of light but is dependent on the products of the light reaction, i.e., ATP and NADPH, besides CO2 and H2O
  • Dark reaction was first of all established by F.F. Blackman (1905) and later on studied in detail by Dr. Calvin, Benson and J. Bassham and for this work they were awarded Nobel Prize in 1961. Biosynthetic or dark phase occurs in stroma or matrix of chloroplast, as all the enzymes required for the process are present there.
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